The Effect Of Variations In African Leaf Extract (Vernonia Amygdalina Del.) Concentration In Loose Powder Formulation On The Inhibitory Activity Against Staphylococcus Aureus

Abstract

Herbal plants that have antibacterial properties are African leaf plants (Vernonia amygdalina del). African leaf extract contains active compounds such as flavonoids, tannins and saponins which have the potential to inhibit bacterial growth. This study aims to determine the antibacterial inhibitory power of African leaf extract loose powder preparations on Staphylococcus aureus bacteria which were tested using 3 African leaf extract loose powder formulas, namely F1 (20%), F2 (30%) and F3 (40%) where clindamycin HCl as a positive control comparison and aquadest as a negative control. The subjects in this study were Staphylococcus aureus bacteria which were divided into 3 petri dishes. The method used in this study was the agar diffusion method using Nutrient Agar (NA) medium in testing bacterial inhibition. The results of the study showed that the average diameter of the inhibition zone obtained was F1 (20%) of 13.86 mm, F2 (30%) of 15,7 mm, F3 (40%) of 18.35 mm, clindamycin HCl (K +) of 23.86 mm and aquadest (K-) had no inhibition zone. Based on the results obtained, it can be concluded that loose powder with a concentration of 20% , 30% and 40% can inhibit the growth of staphylococcus aureus bacteria with a strong category.